Microscope calibration using laser written fluorescence
نویسندگان
چکیده
منابع مشابه
Method of calibration of a fluorescence microscope for quantitative studies.
Confocal microscopy is based on measurement of intensity of fluorescence originating from a limited volume in the imaged specimen. The intensity is quantized in absolute (albeit arbitrary) units, producing a digital 3D micrograph. Thus, one may obtain quantitative information on local concentration of biomolecules in cells and tissues. This approach requires estimation of precision of light mea...
متن کاملFemtosecond fluorescence dynamics imaging using a fluorescence up-conversion microscope.
Femtosecond fluorescence dynamics imaging microscopy was performed. Femtosecond fluorescence dynamics images were constructed based on the "mean" fluorescence decay or rise time constants that were evaluated by the time-resolved intensity sampling using a fluorescence up-conversion microscope. This dynamics imaging microscopy was carried out for the organic microcrystals alpha-perylene and tetr...
متن کاملFluorescence Talbot microscope using incoherent source.
Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15 mm × 15 mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence...
متن کاملThe fluorescence microscope
Dutch envoy to the court of France, mentions the Dutch spectacle-maker Hans Jansen and his son Zacharias as inventors of the first compound microscope in 1595. Their microscope had two lenses combined in a tube in which the magnification was varied by altering the distance between the lenses. However, the Jansens are not known to have published any observations through their microscope; instead...
متن کاملCalibration of atomic force microscope cantilevers using piezolevers.
The atomic force microscope (AFM) can provide qualitative information by numerous imaging modes, but it can also provide quantitative information when calibrated cantilevers are used. In this article a new technique is demonstrated to calibrate AFM cantilevers using a reference piezolever. Experiments are performed on 13 different commercially available cantilevers. The stiff cantilevers, whose...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optics Express
سال: 2018
ISSN: 1094-4087
DOI: 10.1364/oe.26.021887